Shiinaha Novel Coronavirus (SARS-CoV-2) Soosaarayaasha iyo alaab-qeybiyeyaasha PCR-waqtiga dhabta ah |Yinye

Novel Coronavirus (SARS-CoV-2) Qalabka PCR-waqtiga-dhabta ah ee Multiplex

Novel Coronavirus (SARS-CoV-2) Qalabka PCR-waqtiga-dhabta ah ee Isku-dhafka ah ee Sawirka leh

Sharaxaad Gaaban:

REF 500190 Tilmaamid 96 Tijaabooyin/Sanduuqa

Mabda'a ogaanshaha PCR Muunada Sanka / sanka sanka suufka

Faahfaahinta Alaabta

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Qalabkan PCR ee xasaasiga ah, diyaar u ah in la isticmaalo ayaa lagu heli karaa qaab lyophilized ah (habka qalajinta qaboojinta) kaydinta muddada-dheer.Qalabka waxa lagu rari karaa oo lagu kaydin karaa heerkulka qolka waana mid deggan muddo hal sano ah.Tuubo kasta oo premix ah waxa ay ka kooban tahay dhammaan reage-yaasha looga baahan yahay kor u qaadida PCR, oo ay ku jiraan Reverse-transcriptase, Taq polymerase, primers, probes, iyo dNTPs substrates.Kaliya waxay u baahan tahay in lagu daro 13ul biyo nadiif ah iyo 5ul oo la soo saaray RNA template, ka dib waa la ordi karaa oo lagu kordhin karaa qalabka PCR.

Mashiinka qPCR waa inuu la kulmoshuruudaha soo socda:

1. Taam 8 xariijimo PCR mugga tuubada 0.2 ml
2. Hayso in ka badan afar kanaal oo lagu ogaanayo:

Kanaalka	Kacsan (nm)	Sii daynta (nm)	Midabka hore loo hagaajiyay
1.	470	525	FAM, SYBR Cagaaran I
2	523	564	VIC, HEX, TET, JOE
3.	571	621	ROX, TEXAS-RED
4	630	670	CY5

PCR-platforms:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Nidaamka ogaanshaha PCR-waqtiga-dhabta ah, Stratagene Mx3000P, Mx3005P

Dhibaatada gaadiidka silsilad qabow eeNovel Coronavirus nucleic acid reagent ogaanshaha

Marka reagents-ka ogaanshaha aashitada nukliyeerka ee caadiga ah lagu qaado masaafo dheer, (-20 ± 5) ℃ kaydinta silsiladda qabow iyo gaadiidka ayaa loo baahan yahay si loo hubiyo bioactive-ka enzyme ee reagents-ku inay firfircoon yihiin.Si loo hubiyo in heerkulku gaadho heerka, dhawr kiilogaram oo baraf qalalan ayaa loo baahan yahay sanduuq kasta oo ah reagent tijaabinta aashitada nucleic-ka ah xitaa wax ka yar 50g , laakiin waxay socon kartaa oo keliya laba ama saddex maalmood.Marka la eego dhinaca ku dhaqanka warshadaha, miisaanka dhabta ah ee reagents ee ay soo saaraan warshaduhu waa in ka yar 10% (ama wax ka yar qiimahan) weelka.Miisaanka intiisa badan wuxuu ka yimaadaa baraf qallalan, baraf baraf ah iyo sanduuqyada xumbo, markaa qiimaha gaadiidku aad buu u sarreeyaa.

Bishii Maarso 2020, COVID-19 wuxuu bilaabay inuu si baaxad leh ugu dillaaco dibedda, iyo baahida loo qabo in la ogaado Novel Coronavirus nucleic acid reagent ayaa si aad ah u kordhay.Inkasta oo qiimaha sare ee dhoofinta reagents ee silsiladda qabow, inta badan soo saarayaasha weli way aqbali karaan sababtoo ah tiro badan iyo faa'iido sare.

Si kastaba ha ahaatee, iyada oo la wanaajiyey siyaasadaha dhoofinta qaranka ee alaabada ka hortagga masiibada, iyo sidoo kale cusboonaysiinta xakamaynta qaranka ee socodka dadka iyo saadka, waxaa jira kordhin iyo hubin la'aan waqtiga gaadiidka ee reagents, taas oo keentay dhibaatooyin caan ah oo badeecado ah oo sababay gaadiidka.Wakhtiga gaadiidka ee la dheereeyey (wakhtiga gaadiidka ee qiyaastii nus bilood ah ayaa aad u badan) waxay keentaa cillado soo noqnoqda marka alaabtu gaadho macmiilka.Tani waxa ay dhibsatay inta badan reagen-yaasha aashitada nukliyeerka ah ee dhoofiya.

Farsamada Lyophilized ee PCR reagent waxay caawisay gaadiidkaNovel Coronavirus nucleic acid reagent ogaanshaha adduunka oo dhan

Dib-u-hagaajinta PCR-ka lyophilized waa la rari karaa oo lagu kaydin karaa heerkulka qolka, taas oo aan yarayn karin kharashka gaadiidka, laakiin sidoo kale ka fogaanaya dhibaatooyinka tayada ee ay keento habka gaadiidka.Sidaa darteed, lyophilizing reagent waa habka ugu wanaagsan ee lagu xallin karo dhibaatada gaadiidka dhoofinta.

Lyophilization waxay ku lug leedahay qaboojinta xalka xaalad adag, ka dibna hoos u dhig oo kala saar uumiga biyaha ee xaalad faaruq ah.Solubka qallalan wuxuu ku haraa weelka oo leh isku hal abuur iyo waxqabad.Marka la barbar dhigo reageniyeyaasha dareeraha caadiga ah, qayb ka kooban lyophilized Novel Coronavirus nucleic acid reagent oo ay soo saartay Liming Bio waxay leedahay sifooyinka soo socda:

Degganaanshaha kulaylka aadka u xooggan:waxay ku istaagi kartaa daawaynta at 56 ℃ 60 maalmood, iyo qaab-dhismeedka iyo waxqabadka reagent weli waxba iskama beddelin.
Kaydinta heerkulka caadiga ah iyo gaadiidka:Looma baahna silsilad qabow, looma baahna in lagu kaydiyo heerkul hoose ka hor inta aan la furin, si buuxda u sii daa booska kaydinta qabow.
Diyaar u ah in la isticmaalo:lyophilizing ee dhammaan qaybaha, looma baahna qaabeynta nidaamka, iska ilaalinta luminta qaybaha leh viscosity sare sida enzyme.
Bartilmaameedyada isku dhafan ee hal tuubo:Bartilmaameedka ogaanshaha wuxuu daboolayaa coronavirus-ga cusub ee ORF1ab hidda-wadaha, hidda-wadaha N, hiddaha S si looga fogaado genovariation-ka fayraska.Si loo yareeyo diidmada beenta ah, hiddo-wadaha RNase P ee bini'aadamka waxaa loo isticmaalaa xakameyn gudaha ah, si loo daboolo baahida bukaan-socodka ee xakamaynta tayada muunadda.