Novel Coronavirus (2019-nCoV) Qalabka Ogaanshaha Ashitada Ribonucleic (PCR-waqtiga-dhabta ah - Baaritaanka Fluorescent Probe Assay)
La damacsan yahayIsticmaal
Alaabtan waxaa loogu talagalay in lagu ogaado tayada coronavirus-ka cusub (SARS-CoV-2) ee samballada neefsashada.Natiijooyinka ogaanshaha waxaa loo isticmaali karaa in lagu caawiyo ogaanshaha kiliinikada ee Cudurka Coronavirus 2019 (COVID-19).
Qaybaha
2019-nCoV PCR Buffer - 96 imtixaan
Isku-dhafka Enzyme PCR - 96 tijaabo
2019-nCoV Control Togan - 48 imtixaan
2019-nCoV Xakamaynta Xun - 48 imtixaan
2019-nCoV Xakamaynta Gudaha - 96 imtixaan
Gelida Xidhmada - 1 koobi
AlaabtaFarsamaynta
Alaabtani waxay ka kooban tahay afar lammaane oo ah aasaasayaasha PCR, afar shaybaarrada fluorescent, RNA reverse transcriptase, DNA polymerase, dNTP, magnesium ion, iyo kiimikooyin kale oo loogu talagalay ogaanshaha fayraska RNA.Waxay beegsataa hidda-wadaha RNA-ku-tiirsanaanta RNA polymerase (RdRp), hidda-wadaha Nucleocapsid (N), iyo Envelop (E) hidda-wadaha 2019-nCoV isku mar hal tuubo oo tijaabo ah.Habka imtixaanku waxa uu ku socdaa saddexda tallaabo ee soo socda: 1) Dib-u-u-tarjum fayras RNA una beddelo DNA-da fayraska.2) Kordhi DNA-da fayraska ilaa xad la qiyaasi karo.3) Ka warbixi tirada amplicon-yada DNA-ga iyada oo la adeegsanayo isku-dhafka baaritaanka.Ka sokow, alaabtan waxa kale oo ka mid ah xakamaynta gudaha iyo kontoroolka dibadda si loo kormeero waxqabadkeeda inta lagu jiro gaadiidka iyo kaydinta.
